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Image Search Results
Journal: International Journal of Molecular Sciences
Article Title: Normoxic Tumour Extracellular Vesicles Modulate the Response of Hypoxic Cancer and Stromal Cells to Doxorubicin In Vitro
doi: 10.3390/ijms21175951
Figure Lengend Snippet: Overview on the molecular changes related to the resistance inducing mechanisms in C26 cells to DOX treatment under normoxia. Cropped Western blot images and their representative graphs displaying the percentage of protein levels after 24 h treatment with 0.3 µM DOX under normoxia compared to controls (the levels of the same proteins in untreated cell lysates) are shown for phosphatidylinositol-3 kinase (PI3K) in panel ( A ); for protein kinase B (Akt) in panel ( B ); for proto-oncogene tyrosine-protein kinase Src (c-Src) in panel ( C ); for the c-Jun subunit of AP-1 transcription factor (AP-1 c-Jun) in panel ( D ); for p65 subunit of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB p65) in panel ( E ); for c-master regulator of cell cycle entry and proliferative metabolism (c-Myc) in panel ( F ); for B-cell lymphoma–extra-large anti-apoptotic protein (Bcl-xL) in panel ( G ), for Bcl-2-associated X protein (BAX) in panel ( H ); and for hypoxia-inducible factor 1-alpha (HIF-1α) in panel ( I ). β-actin was used as loading control. Data were expressed mean ± SD of duplicate measurements from two independent experiments. Unpaired t -test was used for statistical analysis of the data; ns : not significant, p > 0.05; *, p < 0.05; **, p < 0.01, ***, p < 0.001.
Article Snippet: Membranes were incubated with primary antibodies overnight at 4 °C for p65 subunit of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB p65; mouse monoclonal IgG anti-mouse, 1:500 dilution, sc-56735, Santa Cruz Biotechnology, Santa Cruz, CA, USA), c-Jun subunit of activator protein 1 (AP-1 c-Jun; rabbit polyclonal IgG anti-mouse, 1:1000 dilution, sc-45, Santa Cruz Biotechnology), B-cell lymphoma–extra-large anti-apoptotic protein (Bcl-xL; rabbit monoclonal IgG anti-mouse, 1:500 dilution, 2764, Cell Signaling), phosphatidylinositol-3 kinase (PI3K; rabbit polyclonal IgG anti-mouse, 1:1000 dilution, 4292, Cell Signaling, Beverly, MA, USA), protein kinase B (Akt; rabbit monoclonal IgG anti-mouse, 1:1000 dilution, 4691, Cell Signaling), proto-oncogene tyrosine-protein kinase Src (c-Src; rabbit polyclonal IgG anti-mouse, 1:1000 dilution, sc-018, Santa Cruz Biotechnology), c-master regulator of cell cycle entry and
Techniques: Western Blot, Control